Staining:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
Article Title: HUVECs’ Expressway Based on Magnesium Ion Doped Hierarchical Scaffold for Rapid Angiogenesis and Bone Ingrowth
Article Snippet: Delayed union or nonunion remains an extremely challenging predicament even after the application of currently available engineering scaffolds for the treatment of critical-sized bone defects.. The angiogenesis throughout the artificial bone repair scaffold to achieve “angiogenic-osteogenic coupling” is regarded as an effective approach to solving this issue.. The newly formed blood vessels can accelerate the supply of oxygen and nutrients, thereby triggering subsequent bone regeneration.
Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..
Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).
Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.
Cell Characterization:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
TUNEL Assay:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
Immunohistochemistry:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
Membrane:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
Activity Assay:Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..
other:Article Title: Faecalibacterium prausnitzii as a potential Antiatherosclerotic microbe.
Article Snippet: After washing with PBS the next day, the samples were incubated with fluorescent secondary antibodies at room temperature for 1 h. The nuclei were stained with ready-to-use DAPI (#C0065, Solarbio, China) and placed in PBS at room temperature for 3 min, after which images were collected via microscopy.
Incubation:
Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).
Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.
Double Staining:
Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).
Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.
Saline:Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..
Cytotoxicity Assay:Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..
Bacteria:Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).
Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.
Cell Culture:Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).
Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues.
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